Scale Size Exclusion Chromatography With sEV500 and the ExoStation
The ExoStation and sEV 500 Ultrapure Columns can effectively isolate small extracellular vesicles from samples, and are suitable for the isolation and purification of small extracellular vesicles (sEV) from most biological samples (plasma, serum, urine, etc.) and cell cultures, featuring high purity, high recovery and high throughput. Not only can the total amount of sEV obtained from the purification of 2mL plasma samples meet the requirements of whole group testing, but also help to standardize the operation of sEV isolation and purification in multi-center clinics.
Scale Size Exclusion Chromatography With sEV500 and the EXOSATION
sEV 500 isolation is based on principles of size exclusion chromatography, whereby molecules and particles in solution are separated by their size as they pass through a column consisting of porous resin particles. In sEV500 isolation, larger particles are unable to enter pores in the resin, and instead flow through the resin relatively quickly. In contrast, particles smaller than the lower end of the isolation range enter pores in the resin, which slows their journey. Through this size-based separation, EVs and soluble protein are separated to a high degree of resolution, and a purified volume can be obtained.