Efficient Separation:

The sEV device efficiently isolates extracellular vesicles (EVs), ensuring high purity and yield during the separation process.

Quantitative Performance

The sEV device employs a quantitative separation method, allowing for the isolation of EVs within different size ranges and providing reliable quantitative information.

User-Friendly

The device is designed for ease of use, with straightforward operating procedures that do not require complex steps or specialized training, making it suitable for a wide range of laboratory environments.

Preservation of Vesicle Integrity

The design of the EV device aids in maintaining the integrity of extracellular vesicles, preventing damage during the separation process

How it work

Size Exclusion Chromatography (SEC) is a chromatographic technique primarily designed for the separation of molecules based on their size. While SEC is widely used for the analysis of macromolecules, it may not be the method of choice for the isolation of extracellular vesicles (EVs) due to the relatively small size of EVs. However, some modifications can be applied to make SEC more suitable for EV isolation:

1

Sample Preparation

Prepare the sample by removing cellular debris and large particles through centrifugation or filtration. This step helps to prevent clogging of the SEC column
3

Buffer Selection

Use an appropriate buffer that maintains the stability of extracellular vesicles. Commonly used buffers for EV isolation include phosphate-buffered saline (PBS) or HEPES-buffered saline.
4

Column Calibration

Calibrate the SEC column using standards of known molecular weight. This ensures accurate estimation of the size of the separated vesicles
4

Sample Loading

Load the prepared sample onto the SEC column. As the sample moves through the column, smaller extracellular vesicles will take a longer path through the porous matrix
5

Elution and Collection

Collect fractions as they elute from the column. The elution order will be based on the size of the particles, with larger vesicles eluting earlier.
6

Characterization

Characterize the collected fractions using appropriate methods such as nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), electron microscopy, or other techniques to confirm the presence and size distribution of extracellular vesicles

It's important to note that while SEC can be adapted for EV isolation, there are dedicated methods such as ultracentrifugation, density gradient centrifugation, and commercial kits specifically designed for efficient EV isolation. Researchers should carefully choose the method based on their specific requirements and the nature of the sample. Additionally, maintaining the integrity of extracellular vesicles during the isolation process is crucial for accurate downstream analysis.